The Waters 486 tunable absorbance detector is a single-Channel tunable ultraviolet/visible (UV/VIS) detector designed for high performance liquid chromatography (HPLC) applications.
The Waters 486 tunable absorbance detector can operate as a stand-alone unit (with a chart recorder) or with other Waters system modules, such as the Waters 860 Networking Computer System, or the Water 745/745B or 746 integrators.
The waters 486 tunable absorbance detector provides a usable light intensity over the wavelength range of 190-600 nanometers (nm), Although its primary use is in the ultraviolet wavelength range between 190 and 380 nanometers. The maximum sensitivity of the instrument is 0.001 absorbance units full scale (AUFS).
Wavelength range and sensitivity The Waters 486 tunable absorbance detector provides a usable light intensity over the wavelength range 190 to 600 nanometers (nm), although its primary use is in the ultraviolet range between 190 and 380 nm. The maximum sensitivity of the instrument is 0.001 absorbance units full scale (AUFS).
The Waters 486 tunable absorbance detector offers the following features:
Easy to use operator interface
Self-calibration to ensure wavelength accuracy
Battery backup to retain parameter settings when the detector is powered down or during power interruptions
A deuterium arc lamp
The patented Taper-Cell flow cell design, which reduces interference caused by refractive index effects.
High-pressure (optimized for operation in LC/MS) Lamp shut-off to lengthen usable lamp life
User-selectable chart mark on IV integrator output (standard on 10 mV)
Wavelength scanning to determine optimal wavelength
Diagnostic routines to verify proper functioning
A modular design for easy servicing
Wavelength range (single, fixed wavelength): 190 to 600 nm
Wavelength accuracy: ± 2 nm
Wavelength repeatability: ± 0.25 nm
Noise: ± 1.0 x 10-5 AU, 1 second filter setting, 210 to 280 nm) dry cell
1.0 x 10-4 AU per °C (after warm-up)
1.0 x 10-4 AU per hour (after warm-up)
Sensitivity Setting Range: 0.001 to 2.0 AUFS (under software control)
Filter Setting Range: 0.1 to 5.0 seconds (under software control)
Bandwidth: 8 nm
Optical Component Specifications
Lamp Source: Deuterium arc lamp
Flow cell: Dual beam Taper-Cell design
Path length: 10 mm (standard, analytical)
Cell volume: 8 ul (standard, analytical)
Pressure limit: 1000 psi
Materials: 316 stainless steel, Dynasil Suprasil II, Teflon
Operating temperature: 15 to 32.2 °C
Operating humidity: 20 to 80%, non-condensing
Shipping and storage temperature: -40° to 65 °C
Shipping and storage humidity: 20 to 80% non-condensing