The micrOTOF™ provides powerful exact mass measurements in an easy-to-use benchtop package. Featuring excellent mass resolution and mass accuracy in combination with true isotopic pattern, micrOTOF is the perfect choice for straight-forward formula determination of small molecules, quality control of intact proteins, and metabolic studies.
Superior mass accuracy Superior mass performance characteristics are achieved by combining Bruker Daltonics’ successful time-of-flight (TOF) technology with innovative detection technologies. The micrOTOF shows a mass accuracy of better than 3 ppm without sacrificing resolution or sensitivity. A mass accuracy of better than 5 ppm can be achieved with external calibration without any suppression effects of calibrants and while maintaining the high duty cycle.
TIP™ – True Isotopic Pattern with sigma fit algorithm Replacing the former approach of just using the measured mass for formula determination, the micrOTOF adds a second dimension into the routine: Analysis of the isotope profile provides the so-called “True Isotopic Pattern” (TIP) which is matched against the measured spectrum – leading to results with significantly increased confidence.
Unsurpassed resolution Featuring a mass resolution exceeding 10,000 FWHM (exceeding 15,000 with micrOTOF focus), the micrOTOF defines the new standard in ESI-TOF mass spectrometry. True isotopic resolution can be observed even for deconvoluted spectra at high resolution. The simulated pattern fits perfectly to the measured results.
Sophisticated micrOTOF technology
World-leading combination of mass accuracy, resolution, and sensitivity
Detection of ions with True Isotopic Pattern (TIP™) and Sigma Fit™
Automated determination of elemental composition, the “formula finder”
Footprint 640 x 640 mm, height 1220 mm, weight 130 kg
Standard mass range 50 – 3,000 m/z
Extended mass range 50 – 20,000 m/z
Mass resolution >10,000 FWHM in standard configuration and >15,000 FWHM with micrOTOF focus
Mass accuracy of 3 ppm (RMS) error with internal calibration
Mass accuracy of 5 ppm (RMS) error with external calibration
Acquisition rate of 20 Hz (full mass range profile mass spectra to hard disk)
Polarity switching for both, positive and negative ions
ESI orthogonal electrospray source (1µl –1 ml/min)
APCI orthogonal atmospheric pressure chemical ionization source (100µl –1,5 ml/min)
APPI Atmospheric pressure photo ionization source
AP MALDI Atmospheric pressure MALDI source
Online NanoElectrospray source for capillary LC applications, flow down to 50 nL/min
Offline NanoElectrospray source, consumption of 1–2 µL per sample @ 25 nL/min