Bruker’s microplate extensionHTS-XTallows the application of FTIR spectroscopy as technique for high-throughput screening. The acquisition, control and assessment of spectral data from numerous samples are performed by the OPUS/LAB software. The HTS-XT is an external module that can be linked to various Bruker Optics FTIR spectrometers.
Depending on its configuration, it can be used for measurements ranging from the mid infrared to the near infrared and even the VIS range.
The sampling plates for the IR analysis correspond to the standardized 96- or 384-well microplate formats. For analysis, approximately 1 - 20 µl of a liquid sample is placed on a single sampling position of the microplate and dried. The prepared sample volume depends on measurement mode, sample form, and sample plate design.
First, solid samples are dried and ground then they are filled into a well of the microplate. The loaded sample plate is moved into the sealed optics by a motorized drawer. Next, the measurement of the microplate is conducted fully automated in the measurement modes transmission and/or reflectance by consecutively moving each sample position into the focus of the IR beam.